Peptide Guides: Injection Sites, Reconstitution, Storage, and Basics
PepTrac's peptide guides are five plain-language pages on the practical questions people ask most: where subcutaneous injections go, how reconstitution math works, what bacteriostatic water is, how to store vials and pens, and what a peptide is. Each cites FDA-approved labels, the CDC, and published studies. None of them gives dosing advice.[1]
All guides and trackers
- TrackerPeptide trackerSix records a peptide log needs, and how PepTrac keeps them.
- TrackerPeptide cycle trackerHow on days, off days and dose steps are counted.
- TrackerPeptide stack trackerSeveral compounds, vials and schedules in one weekly view.
- TrackerBPC-157 trackerWhat a BPC-157 log holds, and the evidence gaps behind it.
- TrackerTB-500 trackerA TB-500 dose log, and why its level curve is wide.
- TrackerGLP-1 trackerWeekly shots, sites, weight and protein in one iPhone log.
- TrackerSemaglutide trackerOzempic and Wegovy doses, step-ups and levels on iPhone.
- TrackerTirzepatide trackerMounjaro and Zepbound doses, late shots and levels on iPhone.
- TrackerRetatrutide trackerRetatrutide status, trial facts and what a log can hold.
- GuideWhat are peptides?Definition, peptide vs protein, and FDA status.
- GuideHow to reconstituteTen steps and the math for mixing a dry vial.
- GuideBacteriostatic waterWhat bac water is, how it differs from sterile water.
- GuideHow to store peptidesLabel storage limits by product, and what is unknown.
- GuideInjection sitesLabel-named sites, why rotation matters, how to log it.
- ComparisonBest GLP-1 tracker appsOur pick, and six apps compared feature by feature.
- ComparisonShotsy alternativeWhat PepTrac adds to a Shotsy routine, row by row.
Which peptide guide should I read first?
Start with the guide that matches the question in front of you. If the word peptide is new, read what peptides are first. It uses the National Human Genome Research Institute definition, a short chain of amino acids, typically 2 to 50, joined by peptide bonds, and it separates approved medicines such as Ozempic from products that have no FDA approval.[4] If you are asking how to mix a dry vial, read the reconstitution guide, then the bacteriostatic water guide for the diluent. If you are asking how long something keeps, read the storage guide, which lines up label limits for several products. If you are asking where on the body an injection goes, read the injection sites guide, which compares the abdomen, thigh, and upper arm named on the Ozempic label.[1] Every guide ends with its sources, so you can check each claim yourself.
- I am asking what a peptide is
- Read What are peptides?.
- I am asking how to mix a vial
- Read How to reconstitute peptides, then Bacteriostatic water.
- I am asking how long something lasts
- Read How to store peptides.
- I am asking where to inject
- Read Peptide injection sites.
What does each PepTrac peptide guide cover and cite?
There are five guides, and each one is built on named primary sources instead of opinion. The injection sites guide compares three FDA drug labels, the Ozempic, Wegovy, and Zepbound prescribing information on DailyMed, and adds a 2016 consensus paper on injection technique from Mayo Clinic Proceedings. The reconstitution guide works through the arithmetic and draws its technique steps from the Egrifta WR label, an FDA-approved peptide that ships as a powder. The bacteriostatic water guide rests on the manufacturer label and the CDC rule for opened multi-dose vials.[2] The storage guide sets four product labels side by side and says plainly where no label exists. The basics guide uses the NIH glossary and FDA pages.[4] Because many research peptides have no FDA label, the guides state when a figure is not established and leave the number out rather than guess.[3]
| Guide | Question it answers | Main sources |
|---|---|---|
| Peptide injection sites | Where do subcutaneous injections go, and why rotate? | Ozempic, Wegovy, and Zepbound labels; Mayo Clinic Proceedings 2016 |
| How to reconstitute peptides | How do I mix a dry vial and read the syringe? | Egrifta WR label; bacteriostatic water label; CDC injection safety |
| Bacteriostatic water | What is it, and what is the 28-day rule? | Hospira label on DailyMed; CDC; CDC MMWR 1982 |
| How to store peptides | What do labels say about fridge, freezer, and time? | Ozempic, Wegovy, Zepbound, and Egrifta WR labels; FDA |
| What are peptides? | What is a peptide, and which are FDA-approved? | NHGRI glossary; Federal Register; FDA compounding pages |
How are PepTrac guides researched and sourced?
Guides are written by the PepTrac Editorial Team from primary sources first. That means FDA-approved prescribing information on DailyMed, pages from the FDA and CDC, and peer-reviewed studies found through PubMed. Marketing pages from peptide sellers are never used as evidence. Each factual sentence carries a numbered citation that links to the source list at the bottom of the page, and each source shows the date we last opened it. Every page shows a published date and an updated date, and the updated date changes only when the content changes. No medical professional reviews these pages, and we do not claim one does. If you find a mistake, email team@peptrac.app and we will check it against the source. The full process, including how we handle thin evidence, is on the editorial policy page. The CDC page on safe injection practice is a typical example of the sources used.[2]
Do these peptide guides give dosing or medical advice?
No. The guides explain technique and arithmetic, then point you to the product label, your pharmacist, and your prescriber. They never tell you what to take, how much, or how often. Worked examples are labeled as arithmetic, not as suggested doses. An important reason is that many peptides sold online for research are not FDA-approved. The FDA says compounded drugs are not FDA-approved and that it does not review them for safety, effectiveness, or quality, and it has warned about products falsely labeled for research purposes.[3] The FDA has also listed safety concerns for BPC-157 in compounding, including possible immunogenicity and peptide-related impurities.[5] An FDA-approved product comes with its own label, and that label always outranks a general guide like this one. When your label and a PepTrac page disagree, follow the label and ask your pharmacist.
What is the difference between a guide, a calculator, and a tracker?
A guide explains. It is a written page with sources, such as how reconstitution works or what the label says about storage. A calculator is a free web tool that does one piece of arithmetic on numbers you type in, such as turning vial milligrams and water milliliters into syringe units. It needs no account and never picks a dose. A tracker is the PepTrac iPhone app, which keeps your own record over time. In the app, the Track tab shows each vial's fill level and days left, the Calculator tab works out syringe units, and you record an injection site with each logged dose. PepTrac is a free download with an optional PepTrac Pro subscription, it is published by Blanket LLC, and it does not sell peptides. All of these tools are educational record-keeping, not medical advice.[1]
Frequently asked questions
Are the PepTrac guides free?
Yes. The guides and the web calculators are free to read and use, with no account needed. The PepTrac iPhone app is a free download with an optional PepTrac Pro subscription.
Who writes the guides?
The PepTrac Editorial Team writes them, and PepTrac is published by Blanket LLC. No individual author is named and no medical reviewer is claimed. The editorial policy page explains the process.
How often are the guides updated?
Each page shows a published date and an updated date. We change the updated date only when the page content actually changes, so the date tells you when the information was last revised.
Does PepTrac sell peptides?
No. PepTrac is a record-keeping app and a set of educational pages. It is not a pharmacy and it does not sell, prescribe, or supply peptides or any other medication.
I found an error. How do I report it?
Email team@peptrac.app with the page address and the source that shows the problem. We review each report, correct confirmed errors, and update the page date when we do.
Sources
- DailyMed, National Library of Medicine (Novo Nordisk). Ozempic (semaglutide) injection prescribing information. Accessed October 3, 2026.
- Centers for Disease Control and Prevention. Clinical Safety: Preventing Unsafe Injection Practices. Accessed October 3, 2026.
- U.S. Food and Drug Administration. FDA's Concerns with Unapproved GLP-1 Drugs Used for Weight Loss. Accessed October 3, 2026.
- National Human Genome Research Institute. Talking Glossary of Genomic and Genetic Terms: Peptide. Accessed October 3, 2026.
- U.S. Food and Drug Administration. Certain Bulk Drug Substances for Use in Compounding that May Present Significant Safety Risks. Accessed October 3, 2026.